Beads and other ornaments first appeared during the Palaeolithic period; their small size allowed them to travel considerable distances. Beads also convey cultural, symbolic, and social information. The current paper presents a late 6th–early 5th-millennium BC assemblage of fluorapatite beads, ornaments, and raw material chunks from Tel Tsaf, Jordan Valley, Israel. It discusses their chemical composition, possible sources, distribution, and morphometric and technological characteristics. The results suggest that the raw material for these beads and ornaments derives from the Hatrurim and Daba-Siwaka complexes. While most of the outcrops of these complexes are in the Mottled Zone around the Dead Sea, two additional sources are located closer to Tel Tsaf and near two contemporary sites in Jordan. Although we could not assign the fluorapatite items to a specific source, they may offer the first evidence, beyond the typical Tel Tsaf decoration motifs, for contact between the region’s Middle Chalcolithic sites.