Analysis of the elemental composition of glass has gained traction over the past few decades. The growing interest and utilization of non-destructive and micro-destructive analytical techniques has allowed for a more in-depth understanding of glass production, distribution, and consumption. The analysis of glass trade beads in particular has led to the development of a chronological sequencing for non-diagnostic seed beads opacified with metal oxides as well as ore sourcing for cobalt-blue and red beads. There is deficient research on 18th-century glass bead composition, including black manganese-colored beads. This article explores the elemental composition of 162 black seed beads from three 18th-century sites in Pensacola, Florida, and compares the assemblage to a small sample of similar glass beads recovered from three sites in the United States as well as four potential glass production locations in Europe.